Détail de la notice
Titre du Document
STRATEGIES FOR THE PRODUCTION OF MAIZE-DERIVED PHARMACEUTICALS USING CYTOPLASMIC MALE STERILE LINES: IN VITRO TISSUE CULTURE/TRANSFORMATION AND FIELD BREEDING APPROACHES
Auteur(s)
WANG K. ; FRAME B. ; XU X. ; ...
Corporation(s) du ou des auteurs
European Association for Research on Plant Breeding (EUCARPIA) Maize and Sorghum Section EUR (Organisateur de congres)
Résumé
Plant-made pharmaceuticals (PMPs) offer promise as efficient and cost-effective products for the treatment of human and animal diseases. An advantage of producing pharmaceuticals in maize is the large storage capacity and stability for proteins and starch in seed, allowing for manufacturing recomhinant proteins such as antigens and antibodies. Other advantages of the maize system include safety, high yields, and scalability of production and processing. However, the henefits of this technology must be balanced against potential health and environmental risks that may be associated with its use. Because PMPs presently have no provision for regulatory tolerance, their inadvertent occurrence in foods and feeds remains an important economic consideration, even when the health and environmental risks are low. Pollen drift is considered a source of potential contamination of maize-made pharmaceuticals in the food chain. In addition to physical and temporal isolation requirements, open field pharmaceutical maize production also calls for controlled pollen release. Here, we describe two strategies to address the issue of transgenic pollen drift. First, we describe the development and genetic transformation of a tissue culture-amenable male-sterile line using biolistic- or Agrobacterium-mediated transformation methods. Secondly, we describe the introgression of a transgene from male-fertile transgenic maize to male-sterile germplasm by conventional breeding. After six seasons of breed
Editeur
Maydica
Type du document
Conférence : EUCARPIA Maize and Sorghum International Conference, 21, Bergamo, ITA, 2009-06-21
Identifiant
ISSN : 0025-6153 CODEN : MYDCAH
Source
Maydica A. 2009, vol. 54, n° 2-3, pp. 199-210 [12 pages] [bibl. : 1 p.1/2]
Langue
Anglais
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