Détail de la notice
Titre du Document
Stability of isolated algal chloroplasts that participate in a unique mollusc/kleptoplast association
Auteur(s)
GREEN Brian J. ; FOX Theodore C. ; RUMPHO Mary E.
Résumé
The mollusc Elysia chlorotica Gould has acquired the ability to sustain itself photoautotrophically by forming a symbiotic or kleptoplastic association with chloroplasts of the heterokont alga Vaucheria litorea C. Agardh. The captured chloroplasts (kleptoplasts) remain intact and functional in direct contact with the animal cytosol for up to ten months with no detectable remnant of the algal nucleo-cytosol present. To determine if V. litorea chloroplasts display unusual stability or robustness, isolated plastids were characterized over several days. Structural integrity was physically evaluated by phase-contrast microscopy using Image J software analysis and chemically, by measuring ferricyanide-dependent oxygen evolution. Light-dependent fixation of 14CO2 and in vitro translation of chloroplast proteins were used to measure stroma-dependent functional stability. Spinach chloroplasts were used for comparison. It is concluded that V. litorea chloroplasts are more robust than typical land plant chloroplasts and this robustness may facilitate survival during incorporation of the plastids into the animal cytosol and contribute to their long-term functioning in the foreign cytosol.
Editeur
Balaban; Balaban
Identifiant
ISSN : 0334-5114 CODEN : SYMBER
Source
Symbiosis (Philadelphia, PA) A. 2005, vol. 40, n° 1, pp. 31-40 [10 pages] [bibl. : 1 p.1/2]
Langue
Anglais
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