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Location and timing of initial osteoid deposition in postmeningitic labyrinthitis ossificans determined by multiple fluorescent labels
TINLING Steven P. ; COLTON J. ; BRODIE Hilary A.
Objectives/Hypothesis: Variable amounts of fibrosis and neo-ossification fill the cochlea following bacterial meningitis. The purpose of the study was to delineate the timing and location of initial ossification following pneumococcal meningitis, as well as subsequent remodeling and resorption, over the 3-month period after infection. Study Design: Randomized, double-blind study. Methods: Fluorochromes are compounds that specifically incorporate into ossifying bone. Sequential addition of different colored fluorochromes during osteoneogenesis define the timing and location of osteoid deposition and mineralization. Mongolian gerbils were infected by intrathecal injection of Streptococcus pneumoniae type 3, and control gerbils received saline. Both groups were injected with calcein on postoperative day 3, followed by xylenol orange, oxytetracycline, and alizarin red on days 7, 14, and 28 respectively. Ten experimental gerbils were killed 24 hours after each label, and an additional group at 84 days after infection. Two groups of 10 control gerbils were killed at 29 and 84 days after treatment. The temporal bones and tibias were harvested, embedded in plastic, and sliced with a diamond saw. Wafers at a thickness of 200 pm were mounted in sequence and examined. Results: Sixteen of 49 experimental animals (33%) were positive for at least one of the fluorescent labels. Fluorescent labeled osteoid was present at all sampling times. Label extended from the endosteal wall into the lum
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Conférence : Midwinter Meeting of the Association for Research in Otolaryngology, 26, Daytona Beach, FL, USA, 2003-02
PMID : 15064623 ISSN : 0023-852X CODEN : LARYA8
The Laryngoscope A. 2004, vol. 114, n° 4, pp. 675-680 [6 pages] [bibl. : 34 ref.]
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