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Titre du Document
Adenine nucleotide and protein kinase C regulation of renal tubular epithelial cell wound healing
Auteur(s)
SPONSEL H. T. ; BRECKON R. ; ANDERSON R. J.
Résumé
Adenine nucleotide and protein kinase C regulation of renal tubular epithelial cell wound healing. The present studies were done to determine the effect of selected adenine nucleotides on healing of wounds made by mechanically denuding areas in confluent monolayers of renal tubular epithelial cells. We found that hydrolyzable and nonhydrolyzable forms of ATP but not UTP stimulated healing of LLC-PK1 cell wounds, while both ATP and UTP promoted healing of MDCK cell wounds, suggesting that different subtypes of purinoceptors regulated wound healing in these cells. Stimulation of wound healing by ATP was equivalent in control cells and in cells in which irradiation suppressed proliferation, suggesting a prominent role for cell migration in the healing process. Since ATP receptors are often linked to activation of protein kinase C, the effect of a protein kinase C activator (4β-phorbol 12-myristate 13-acetate, PMA) on wound healing was studied. Long-term (24 hr) exposure to PMA inhibited while short-term (30-120 min) exposure to PMA enhanced cell wound healing. Two chemically and mechanistically dissimilar protein kinase C inhibitors (calphostin C and chelerythrine) inhibited LLC-PK1 and MDCK cell wound healing, and calphostin C prevented ATP enhancement of LLC-PK, healing. These observations suggest a role for protein kinase C in regulation of basal and adenine nucleotide-stimulated wound healing. Adenosine triphosphate did not affect cell-cell adhesion of either LL
Editeur
Nature Publishing
Identifiant
PMID : 7564096 ISSN : 0085-2538 CODEN : KDYIA5
Source
Kidney international A. 1995, vol. 48, n° 1, pp. 85-92 [bibl. : 42 ref.]
Langue
Anglais
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