Détail de la notice
Titre du Document
Inhibition of human plasma and serum butyrylcholinesterase (EC 3.1.1.8) by α-chaconine and α-solanine
Auteur(s)
NIGG H. N. ; RAMOS L. E. ; GRAHAM E. M. ; ...
Résumé
The purpose of these experiments was to determine the reversibility of α-chaconine and α-solanine inhibition of human plasma butyrylcholinesterase (BuChE). For the substrate α-naphthylacetate, optimal assay conditions were 0.50 M sodium phosphate buffer and a substrate concentration of 3-5 x 10-4 M : Dibucaine (1 x 10-5 M) indicated the usual phenotype for all subjects ; α-chaconine and α-solanine at 2.88 x 10-6 M inhibited BuChE about 70 and 50%, respectively. One- and 24-hr incubations at 1 x 10-5 M with α-chaconine, α-solanine, paraoxon, eserine, and ethanol yielded reversible inhibition with dilution except for paraoxon. Twenty-four-hour dialyses of incubations showed no inhibition except for paraoxon. PAGE enzyme activity gels of 1- and 24-hr incubations also showed no inhibition except for paraoxon. α-Chaconine and α-solanine are reversible inhibitors of human butyrylcholinesterase. At estimated tissue levels, α-chaconine, α-solanine, and solanidine inhibited BuChE 10-86%. In assays which combined α-chaconine, α-solanine, and solanidine, inhibition of BuChE was less than additive. No inhibition of albumin α-naphthylacetate esterase (an arylesterase) was noted with any inhibitor. The importance of these data to adverse toxicological effects of potato alkaloids is discussed
Editeur
Academic Press; Academic Press; Academic Press
Identifiant
PMID : 8921346 ISSN : 0272-0590 CODEN : FAATDF
Source
Fundamental and applied toxicology (Print) A. 1996, vol. 33, n° 2, pp. 272-281 [bibl. : 2 p.1/4]
Langue
Anglais
Pour les membres de la communauté du CNRS, ce document est autorisé à la reproduction à titre gratuit.
Pour les membres des communautés hors CNRS, la reproduction de ce document à titre onéreux sera fournie sous réserve d’autorisation du Centre Français d’exploitation du droit de Copie.

Pour bénéficier de nos services (strictement destinés aux membres de la communauté CNRS (Centre National de la Recherche Scientifique), de l'ESR français (Enseignement Supérieur et Recherche), et du secteur public français & étranger) :