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Characterization of Rhizobium naphthalenivorans sp. nov. with special emphasis on aromatic compound degradation and multilocus sequence analysis of housekeeping genes
KAIYA Shinichi ; RUBABA Owen ; YOSHIDA Naoko ; ...
Three strains of aerobic chemoorganotrophic naphthalene-degrading bacteria (designated TSY03bT, TSY04, and TSW01) isolated from sediment of a polychlorinated-dioxin-transforming microcosm were characterized. These strains had Gram-negative-stained, rod-shaped cells measuring 0.6-0.9 μm in width and 1.2-3.0 μm in length and were motile by means of peritrichous flagella. Naphthalene was utilized as the sole carbon and energy source, and the transcription of a putative aromatic-ring hydroxylating gene was inducible by naphthalene. The major component of cellular fatty acids was summed feature 8 (C18:1ѡ7c and/or C18:1ѡ6c), and significant proportions of C18:0 and C19:0 cyclo ѡ8cis were also found. The major respiratory quinone was ubiquinone-10. The G+C content of the DNA was 60.3-60.9 mol%. Phylogenetic analyses by studying sequence information on the housekeeping atpD, dnaK, glnll, gyrB, and recA genes as well as on 16S rRNA genes and the 16S-23S rDNA internal transcribed spacer region revealed that the strains grouped with members of the genus Rhizobium, with Rhizobium selenitireducens as their closest relative but formed a distinct lineage at the species level. This was confirmed by genomic DNA-DNA hybridization studies. These phenotypic, genotypic, and phylogenetic data strongly suggest that our isolates should be classified under a novel species of the genus Rhizobium. Thus, we propose the name Rhizobium naphthalenivorans sp. nov. to accom
Microbiology Research Foundation
ISSN : 0022-1260 CODEN : JGAMA9
Journal of General and Applied Microbiology A. 2012, vol. 58, n° 3, pp. 211-224 [14 pages] [bibl. : 1 p.3/4]
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