Détail de la notice
Titre du Document
Physiological analysis of transgenic Arabidopsis thaliana plants expressing one nitrilase isoform in sense or antisense direction
Auteur(s)
GRSIC S. ; SAUERTEIG S. ; NEUHAUS K. ; ...
Résumé
The biosynthesis of IAA in Arabidopsis thaliana can proceed via different pathways, which involve indole-3-acetonitrile (IAN) as an intermediate. The enzyme nitrilase, which converts IAN to the auxin indole-3-acetic acid (IAA), might be a key enzyme in this biosynthetic pathway. To elucidate the role of nitrilase during the development of the plant, we have investigated transgenic Arabidopsis plants, carrying one of the four nitrilase isoforms so far identified in Arabidopsis in either sense or antisense direction. The plants have been analyzed for their phenotype, auxin content, nitrilase transcript and protein amounts, and nitrilase activity. Analysis of free IAA content in seedlings did not reveal any prominent differences between the different lines, whereas the total IAA content was slightly increased in the line overexpressing NIT2 (sNIT2). In two of the antisense lines (aN IT 1 and aNIT2) the total auxin content was about 75 % lower than in the wild type, while IAN was increased in these two antisense lines by about 2-fold. In sNIT2 plants the IAN content was slightly decreased compared with the wild type. When IAN was added to the medium, a strong increase in IAA content was found in sNIT2 seedlings (ca. 22-fold), which was much lower in wild type and antisense plants. In mature plants levels of free IAA were decreased by about 25 % and 50% in the antisense lines aNIT2 and aNIT 1, respectively, whereas the IAA content in the other lines did not differ much, sNIT2/aNIT
Editeur
Elsevier
Identifiant
ISSN : 0176-1617 CODEN : JPPHEY
Source
Journal of plant physiology A. 1998, vol. 153, n° 3-4, pp. 446-456 [bibl. : 29 ref.]
Langue
Anglais
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