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Titre du Document
Using pulse field gradient NMR diffusion measurements to define molecular size distributions in glycan preparations
Auteur(s)
MILLER Michelle C. ; KLYOSOV Anatole ; PLATT David ; ...
Résumé
Glycans comprise perhaps the largest biomass in nature, and more and more glycans are used in a number of applications, including those as pharmaceutical agents in the clinic. However, defining glycan molecular weight distributions during and after their preparation is not always straightforward. Here, we use pulse field gradient (PFG) 1H NMR self-diffusion measurements to assess molecular weight distributions in various glycan preparations. Initially, we derived diffusion coefficients, D, on a series of dextrans with reported weight-average molecular weights from about 5 kDa to 150 kDa. For each dextran sample, we analyzed 15 diffusion decay curves, one from each of the 15 major 1H resonance envelopes, to provide diffusion coefficients. By measuring D as a function of dextran concentration, we determined D at infinite dilution, Dinf, which allowed estimation of the hydrodynamic radius, Rh, using the Stokes-Einstein relationship. A plot of log Dinf versus log Rh was linear and provided a standard calibration curve from which Rh is estimated for other glycans. We then applied this methodology to investigate two other glycans, an α-(1→2)-L-rhamnosyl-α-(1→4)-D-galacturonosyl with quasi-randomly distributed, mostly terminal β(1→4)-linked galactose side-chains (GRG) and an α(1→6)-D-galacto-β(1→4)-D-mannan (Davanat), which is presently being tested against cancer in the clinic. Using the dextran-derived calibration curve, we
Editeur
Elsevier
Identifiant
ISSN : 0008-6215 CODEN : CRBRAT
Source
Carbohydrate research A. 2009, vol. 344, n° 10, pp. 1205-1212 [8 pages]
Langue
Anglais
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