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Titre du Document
Telomerase activity and telomere length in pediatric patients with malignancies undergoing chemotherapy
Auteur(s)
ENGELHARDT M. ; OZKAYNAK M. F. ; DRULLINSKY P. ; ...
Résumé
Telomerase activity and telomere length in mononuclear cells (MNCs) and granulocytes from peripheral blood (PB) and bone marrow (BM) specimens were studied in pediatric acute leukemia (ALL, n=15; AML, n=1) and pediatric solid tumor (ST) patients (n = 9) at diagnosis, during and after chemotherapy. In four ST patients, tumor tissue was also available. For comparative analysis, MNCs from healthy donors (n=53) were analyzed. Telomerase was evaluated using a modified telomeric repeat amplification protocol (TRAP) assay, and telomere length by terminal restriction fragment (TRF) analysis. At diagnosis, high telomerase activity was detected in MNCs from all leukemia patients, which was similar to the activity from ST biopsy specimens. This exceeded by 10- to 20-fold the activity in PB MNCs from ST patients and healthy donors (P< 0.05). Granulocyte fractions lacked telomerase activity in all groups. BM MNCs in leukemia patients revealed a four-fold higher telomerase activity than PB (P=0.005). After induction chemotherapy and response to treatment, telomerase activity decreased to borderline or undetectable levels in PB MNCs in leukemia (P < 0.01). Average telomeres in PB MNCs from pediatric patients were significantly longer (n = 25; 10.9 kbp) than telomeres in PB and BM MNCs from adult healthy donors (7.45 kbp) (P< 0.0001). At diagnosis, telomeres were shorter from BM compared to PB specimens In leukemia (P< 0.05), and two peak TRFs were observed corresponding to the malignant and
Editeur
Nature Publishing
Identifiant
PMID : 9436916 ISSN : 0887-6924 CODEN : LEUKED
Source
Leukemia A. 1998, vol. 12, n° 1, pp. 13-24 [bibl. : 64 ref.]
Langue
Anglais
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