Détail de la notice
Titre du Document
Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L
Auteur(s)
REDDY Muntha K. ; GUPTA Sashi K. ; JACOB Melissa R. ; ...
Résumé
The Punica granatum L (pomegranate) by-product POMx was partitioned between water, EtOAc and n-BuOH, and the EtOAc and n-BuOH extracts were purified by XAD-16 and Sephadex LH-20 column chromatography to afford ellagic acid (1), gallagic acid (2), punicalins (3), and punicalagins (4). Compounds 1-4 and the mixture of tannin fractions (XAD-16 eluates) were evaluated for antioxidant, antiplasmodial, and antimicrobial activities in cell-based assays. The mixture of tannins (TPT), XAD-EtOAc, XAD-H2O, XAD-PJ and XAD-BuOH, exhibited IC50 values against reactive oxygen species (ROS) generation at 0.8-19μg/ mL. Compounds 1-4 showed IC50values of 1.1, 3.2, 2.3 and 1.4 pM, respectively, against ROS generation and no toxicity up to 31.25 μg/mL against HL-60 cells. Gallagic acid (2) and punicalagins (4) exhibited antiplasmodial activity against Plasmodium falciparum D6 and W2 clones with IC50values of 10.9,10.6, 7.5 and 8.8 μM, respectively. Fractions XAD-EtOAc, XAD-BuOH, XAD-H20 and XAD-PJ compounds 1-4 revealed antimicrobial activity when assayed against Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Cryptococcus neoformans, methicillin-resistant Staphylococcus aureus (MRSA),Aspergillus fumigatus and Mycobacterium intracellulare. Compounds 2 and 4 showed activity against P. aeruginosa, C. neoformans, and MRSA. This is the first report on the antioxidant, antiplasmodial and antimicrobial activities of POMx isolates, including structure-activity relations
Editeur
Thieme; Thieme
Identifiant
PMID : 17566148 ISSN : 0032-0943 CODEN : PLMEAA
Source
Planta medica A. 2007, vol. 73, n° 5, pp. 461-467 [7 pages] [bibl. : 30 ref.]
Langue
Anglais
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