Détail de la notice
Titre du Document
Immunotherapeutic effect of concholepas hemocyanin in the murine bladder cancer model : Evidence for conserved antitumor properties among hemocyanins
Auteur(s)
MOLTEDO Bruno ; FAUNES Fernando ; HAUSSMANN Denise ; ...
Résumé
Purpose: We determined the antitumor properties of a newly available hemocyanin obtained from the Chilean gastropod Concholepas concholepas (Biosonda Corp., Santiago, Chile) in a syngeneic heterotopic mouse bladder carcinoma model. Since keyhole limpet hemocyanin (Pierce, Rockford, Illinois) is used increasingly in biomedicine as a carrier for vaccines and an immunotherapeutic agent for bladder transitional cell carcinoma, there is a growing interest in finding new substances that share its potent immunomodulatory properties. Considering that keyhole limpet hemocyanin and Concholepas concholepas hemocyanin differ significantly, it was not possible to predict a priori the antitumor properties of Concholepas concholepas hemocyanin. Materials and Methods: C3H/He mice were primed with Concholepas concholepas hemocyanin before subcutaneous implantation of mouse bladder tumor-2 cells. Treatment consisted of a subcutaneous dose of Concholepas concholepas hemocyanin (1 mg or 100 μg) at different intervals after implantation. Keyhole limpet hemocyanin and phosphate buffered saline served as positive and negative controls, respectively. In addition, experiments were designed to determine which elements of the immune response were involved in its adjuvant immunostimulatory effect. Results: Mice treated with Concholepas concholepas hemocyanin showed a significant antitumor effect, as demonstrated by decreased tumor growth and incidence, prolonged survival and lack of toxic effects. Thes
Editeur
Lippincott Williams & Wilkins
Identifiant
ISSN : 0022-5347 CODEN : JOURAA
Source
The Journal of urology A. 2006, vol. 176, 1, pp. 2690-2695 [6 pages] [bibl. : 20 ref.]
Langue
Anglais
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