Détail de la notice
Titre du Document
Measurement of a genotoxic hydrazine, agaritine, and its derivatives by HPLC with fluorescence derivatization in the Agaricus mushroom and its products
Auteur(s)
NAGADKA Megumi Hamano ; NAGAOKA Hiroaki ; KONDO Kazunari ; ...
Résumé
Agaricus blazei MURRILL mushroom products are sold as socalled health foods in Japan. However, a part of Agaricus is known to contain hydrazines. A sensitive and specific method for analyzing a genotoxic hydrazine, agaritine, and its derivatives was developed to assess the safety of Agaricus products. β-N-(y-L(+)-Glutamyl)-4-(hydroxymethyl)phenylhydrazine (agaritine, AGT), 4-hydrazinylbenzylalcohol (HMPH), 4-hydrazinyl-benzoic acid (CPH), 4-methylphenylhydrazine (MPH) and phenylhydrazine (PH) were converted to their corresponding fluorescent derivatives with 3,4-dihydro-6,7 dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride (DMEQ-COCl) as the fluorescence derivatization reagent. The detection limits (S/N=3) for CPH, AGT, PH and MPH were 422, 45.3, 16.5 and 138fmol, respectively, in a 20 μl injection volume. Recoveries, achieved by adding known AGT amounts to the Agaricus sample and Agaricus products, ranged from 92.8 to 102%. By using this method which does not require partial purification of the Agaricus sample, the amounts of AGT in several types of foods were found to be 112-1836 μg/g dry weight.
Editeur
JAACC
Identifiant
ISSN : 0009-2363 CODEN : CPBTAL
Source
Chemical and pharmaceutical bulletin A. 2006, vol. 54, n° 6, pp. 922-924 [3 pages]
Langue
Anglais
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