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Titre du Document
Angioprotective action of calcium dobesilate against reactive oxygen species-induced capillary permeability in the rat
Auteur(s)
BRUNET J. ; FARINE J.-C. ; GARAY R. P. ; ...
Résumé
Calcium dobesilate possesses antioxidant properties in vitro, but the in vivo significance and putative angioprotective role of these properties are undefined. Here, calcium dobesilate was tested in a newly developed in vivo model of microvascular permeabilization induced by reactive oxygen species in the rat peritoneal cavity. In this model, microvascular permeabilization is equated to the rate of Evans blue extravasation toward the peritoneal cavity. Basal Evans blue extravasation (rate constant values ke = 0.0176 ± 0.0015 h-1) was markedly and significantly increased by reactive oxygen species generated in situ, with: (i) phenazine methosulfate/NADH (Δke(phenazine methosulfate) = 0.0419 ± 0.0043 h-1) and (ii) xanthine/xanthine oxidase (Δke(XO) = 0.0383 ± 0.0010x h- 1). These actions of reactive oxygen species were abolished by locally injected superoxide dismutase (i.p., 300 units/kg). Intraperitoneally given calcium dobesilate (100 mg/kg) inhibited 75-100% of reactive oxygen species-induced Evans blue extravasation. By the intravenous route, calcium dobesilate i.v. (1-50 mg/kg) dose dependently inhibited phenazine methosulfate-induced Evans blue extravasation with an ID50 of 2-5 mg/kg (full inhibition was reached at 20-50 mg/kg). After single oral administration, calcium dobesilate (5-500 mg/kg) dose dependently inhibited phenazine methosulfate-dependent Evans blue extravasation with an ID50 of 50-100
Editeur
Elsevier
Identifiant
PMID : 9822887 ISSN : 0014-2999 CODEN : EJPHAZ
Source
European journal of pharmacology A. 1998, vol. 358, n° 3, pp. 213-220 [bibl. : 38 ref.]
Langue
Anglais
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