Détail de la notice
Titre du Document
Glycosylated compounds from okra inhibit adhesion of Helicobacter pylori to human gastric mucosa
Auteur(s)
LENGSFELD Christian ; TITGEMEYER Fritz ; FALLER Gerhard ; ...
Résumé
In Asian medicine the fruit of the okra plant, Abelmoschus esculentus (L.) Moench., is used as a mucilaginous food additive against gastric irritative and inflammative diseases. To find a rational basis for its use against these diseases, several crude and purified carbohydrate-containing fractions from immature okra fruits were isolated and analyzed, and their effects against Helicobacter pylori in an in situ adhesion model on sections of human gastric mucosa were determined. Pretreatment of the bacteria with a fresh juice preparation inhibited the bacterial adhesion almost completely. Lyophilization and reconstitution of an extract solution led to a reduction of this effect. A crude polysaccharide (RPS) isolated from the fresh juice by ethanolic precipitation showed strong inhibitory effects. Further fractionation of RPS revealed a purified, highly acidic subfraction (AF III) with high antiadhesive qualities. Carbohydrate analysis revealed the presence of rhamnogalacturonans with a considerable amount of glucuronic acid, whereas other inactive subfractions contained little glucuronic acid or were glucuronic acid-free. After heat denaturation of the fresh juice or protein precipitation with 5% TCA the antiadhesive activity of the fresh extract was reduced, indicating that besides polysaccharides, protein fractions also exhibited antiadhesive properties. SDS-PAGE analysis of the precipitate revealed several bands of glycosylated proteins between 25 and 37 kDa that were almost
Editeur
American Chemical Society
Identifiant
PMID : 15030201 ISSN : 0021-8561 CODEN : JAFCAU
Source
Journal of agricultural and food chemistry (Print) A. 2004, vol. 52, n° 6, pp. 1495-1503 [9 pages] [bibl. : 30 ref.]
Langue
Anglais
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