Détail de la notice
Titre du Document
Molecular structures of Fructans from Agave tequilana Weber var. azul
Auteur(s)
LOPEZ Mercedes G. ; MANCILLA-MARGALLI Norma A. ; MENDOZA-DIAZ Guillermo
Résumé
Agave plants utilize crassulacean acid metabolism (CAM) for CO2 fixation. Fructans are the principal photosynthetic products generated by agave plants. These carbohydrates are fructose-bound polymers frequently with a single glucose moiety. Agave tequilana Weber var. azul is an economically important CAM species not only because it is the sole plant allowed for tequila production but because it is a potential source of prebiotics. Because of the large amounts of carbohydrates in A. tequilana, in this study the molecular structures of its fructans were determined by fructan derivatization for linkage analysis coupled with gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR), and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS). Fructans were extracted from 8-year-old A. tequilana plants. The linkage types present in fructans from A. tequilana were determined by permethylation followed by reductive cleavage, acetylation, and finally GC-MS analysis. Analysis of the degree of polymerization (DP) estimated by 1H NMR integration and 13C NMR and confirmed by MALDI-TOF-MS showed a wide DP ranging from 3 to 29 units. All of the analyses performed demonstrated that fructans from A. tequilana consist of a complex mixture of fructooligosaccharides containing principally β(2 → 1) linkages, but also β(2 → 6) and branch moieties were observed. Finally, it can be stated that fructans from A. tequilana Weber var
Editeur
American Chemical Society
Identifiant
PMID : 14690361 ISSN : 0021-8561 CODEN : JAFCAU
Source
Journal of agricultural and food chemistry (Print) A. 2003, vol. 51, n° 27, pp. 7835-7840 [6 pages] [bibl. : 29 ref.]
Langue
Anglais
Pour les membres de la communauté du CNRS, ce document est autorisé à la reproduction à titre gratuit.
Pour les membres des communautés hors CNRS, la reproduction de ce document à titre onéreux sera fournie sous réserve d’autorisation du Centre Français d’exploitation du droit de Copie.

Pour bénéficier de nos services (strictement destinés aux membres de la communauté CNRS (Centre National de la Recherche Scientifique), de l'ESR français (Enseignement Supérieur et Recherche), et du secteur public français & étranger) :