Détail de la notice
Titre du Document
Gc protein-derived macrophage activating factor (GcMAF): Isoelectric focusing pattern and tumoricidal activity
Auteur(s)
SAHARUDDIN BIN MOHAMAD ; NAGASAWA Hideko ; SASAKI Hideyuki ; ...
Corporation(s) du ou des auteurs
Society of Biotherapeutic Approaches INC (Commanditaire)
Résumé
Background: Gc protein is the precursor for Gc protein-derived macrophage activating factor (GcMAF), with three phenotypes: Gc1f, Gcls and Gc2, based on its elecrophoretic mobility. The difference in elecrophoretic mobility is because of the difference in its posttranslational sugar moiety composition. Materials and Methods: We compared the difference between Gc protein and GcMAF elecrophoretic mobility using the isoelectric focusing (IEF) method. The tumoricidal activity of GcMAF-treated macrophage was evaluated after coculture with L-929 cell. The tumoricidal mechanism was investigated using TNF bioassay and nitric oxide (NO) release. Results: The difference in Gc protein and GcMAF elecrophoretic mobility was detected. The tumoricidal activity of GcMAF-treated macrophage was detected, but no release of TNF and NO was detected. Conclusion: The difference of isoelectric focusing mobility in Gc protein and GcMAF would be useful to develop a GcMAF detection method. GcMAF increased macrophage tumoricidal activity but TNF and NO release were not involved in the mechanism.
Editeur
International Institute of Anticancer Research
Type du document
Conférence : Annual Meeting of the Society of Biotherapeutic Approaches, 6, Fukuoka, JPN, 2002-12-07
Identifiant
ISSN : 0250-7005
Source
Anticancer research A. 2003, vol. 23, n° 6A, pp. 4451-4457 [7 pages] [bibl. : 21 ref.]
Langue
Anglais
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