Détail de la notice
Titre du Document
Immunosuppression and oxidative stress induced by acute and chronic exposure to cocaine in rat
Auteur(s)
PACIFICI Roberta ; FIASCHI Anna Ida ; MICHELI Lucia ; ...
Résumé
The objective of the present study was to verify if immunosuppression caused by cocaine (CO) can be mediated, at least in part, by increased formation of oxidative metabolites and reactive oxygen species (ROS) in rat. Pharmacokinetics of cocaine and its metabolites, cell-mediated immune function and cytokines production, biomarkers of cell redox state maintenance and lipidic peroxidation, and variations of activity in the enzymatic systems involved in cell antioxidant defence were measured in spleen of Wistar rats acutely and chronically treated with cocaine. Cmax, AUC, and 11/2 of norcocaine (NC) significantly increased after chronic exposure to cocaine while kinetic parameters of benzoylecgonine (BE) significantly decreased. A decrease in cultured T-lymphocytes proliferation and natural killer (NK) cell activity, a high increase of immunosuppressive cytokines and a switch from Th1-type cytokines to Th2-type cytokines together with an unbalance toward anti-inflammatory cytokines recovered within 4 h after acute treatment while subsisted for 14 days after chronic treatment. A significant increase in ascorbic acid (AA), reduced glutathione and glutathione reductase (GR) with a simultaneous decrease in oxidized glutathione were observed in the first hours after acute administration. Conversely, the increase in oxidized glutathione and malondialdehyde (MDA) production and the simultaneous depletion of reduced glutathione and ascorbic acid persisted at least 24 h afte
Editeur
Elsevier
Identifiant
PMID : 12689662 ISSN : 1567-5769
Source
International immunopharmacology (Print) A. 2003, vol. 3, n° 4, pp. 581-592 [12 pages] [bibl. : 41 ref.]
Langue
Anglais
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