Détail de la notice
Titre du Document
Concept of sequential analysis of free and conjugated phytosterols in different plant matrices
Auteur(s)
BREINHÖLDER Patrick ; MOSCA Livia ; LINDNER Wolfgang ; ...
Résumé
A unique concept and method for the determination of the total plant sterol content as sum of free sterols (FS), steryl esters (SE), steryl glycosides (SG) and acylated steryl glycosides (ASG) in different plant materials (pumpkin seeds. lecithins) and phytopharmaceuticals derived thereof, was developed. For this purpose, a multidimensional sample clean-up protocol based on efficient solid-phase extraction materials was elaborated and the SG were isolated employing a novel phenyl boronic acid modified silica gel material. Along this line also a set of steryl glucosides was synthesised and employed as internal standard and for calibration in the course of quantitative analysis. Final quantification of SG was carried out with reversed-phase HPLC in combination with evaporative light scattering detection (ELSD); the ASG were determined after conversion to SG by mild alkaline hydrolysis. In order to determine the total plant sterol profile the sum of FS and SE was additionally analysed from the unsaponifiable lipid fraction by GC-FID. The yields obtained from recovery tests for the determination of SG using soya lecithin as matrix to which 2, 20 and 40 mg/g of cholesterol-β-D-glucoside was added were 99.10, 98.07 and 90.00%, and the RSDs were 4.11, 2.62 and 4.50%, respectively. Application related to the qualitative and quantitative analysis of total phytosterol profiles in different plant matrices and extracts demonstrate the validity of the method.
Editeur
Elsevier Science
Identifiant
ISSN : 1387-2273
Source
Journal of chromatography. B, Biomedical sciences and applications A. 2002, vol. 777, n° 1-2, pp. 67-82 [16 pages] [bibl. : 25 ref.]
Langue
Anglais
Pour les membres de la communauté du CNRS, ce document est autorisé à la reproduction à titre gratuit.
Pour les membres des communautés hors CNRS, la reproduction de ce document à titre onéreux sera fournie sous réserve d’autorisation du Centre Français d’exploitation du droit de Copie.

Pour bénéficier de nos services (strictement destinés aux membres de la communauté CNRS (Centre National de la Recherche Scientifique), de l'ESR français (Enseignement Supérieur et Recherche), et du secteur public français & étranger) :