Détail de la notice
Titre du Document
Gastrointestinal enzyme production of bioactive peptides from royal jelly protein and their antihypertensive ability in SHR
Auteur(s)
MATSUI Toshiro ; YUKIYOSHI Akiko ; DOI Shima ; ...
Résumé
In order to clarify the potential physiological function of royal jelly (RJ), we report here the gastrointestinal enzyme production of antihypertensive peptides from RJ. Intact RJ and its protein fraction did not retard the action of angiotensin I-converting enzyme (ACE) activity at all. However, development of ACE inhibition power of RJ was newly observed by pepsin hydrolysis (IC50=0.358 mg protein/mL), and the subsequent trypsin and chymotrypsin hydrolyses (IC50=0.099 mg protein/mL). Single oral administration of this gastrointestinal RJ hydrolysate (1 glkg dose) in 10-week spontaneously hypertensive rat resulted in a significant reduction of systolic blood pressure of 22.7 ± 3.6 mmHg at 2 hr (P<0.05 vs. 0 hr by one-way ANOVA, n=7). Then, the RJ hydrolysate was fractionated with gel permeation chromatography to obtain the di- and tri-peptides (DTP) fraction. As a result of isolation from the DTP fraction by reversed phase-high performance liquid chromatography, eleven ACE inhibitory peptides were isolated from the DTP-RJ hydrolysate. Some of the ACE inhibitors were derived from the RJ-glycoprotein; eight peptides with the IC50 value of <10 μM were identified from natural resources for the first time. Consequently, RJ protein was thought to be a good resource of ACE inhibitory peptides produced by the gastrointestinal enzyme hydrolyses.
Editeur
Elsevier Science
Identifiant
ISSN : 0955-2863
Source
Journal of nutritional biochemistry A. 2002, vol. 13, n° 2, pp. 80-86 [bibl. : 32 ref.]
Langue
Anglais
Pour les membres de la communauté du CNRS, ce document est autorisé à la reproduction à titre gratuit.
Pour les membres des communautés hors CNRS, la reproduction de ce document à titre onéreux sera fournie sous réserve d’autorisation du Centre Français d’exploitation du droit de Copie.

Pour bénéficier de nos services (strictement destinés aux membres de la communauté CNRS (Centre National de la Recherche Scientifique), de l'ESR français (Enseignement Supérieur et Recherche), et du secteur public français & étranger) :