Détail de la notice
Titre du Document
Chronic venous insufficiency is associated with increased platelet and monocyte activation and aggregation. Discussion
Auteur(s)
POWELL C. C. ; ROHRER M. J. ; BARNARD M. R. ; ...
Corporation(s) du ou des auteurs
American Venous Forum USA (Commanditaire)
Résumé
Purpose: This study assessed whether the increased numbers of platelet-monocyte aggregates observed in patients with venous stasis ulceration (VSU) represent a response to dermal ulceration or if it is a condition associated with underlying chronic venous insufficiency (CVI). We also analyzed the expression of CD11b in patients with CVI to determine whether leukocyte activation, known to occur in VSU, is a precursor of or a response to ulceration. Methods: Patients with varying classes of CVI (n = 24) and healthy control subjects (n = 15), whose status was documented by means of duplex scanning, stood upright and stationary for 10 minutes. Two aliquots of blood, drawn from a distal leg vein and an antecubital fossa vein, were incubated with either buffer or one of three platelet agonists. After fixation, these samples were further incubated with fluorescent-labeled monoclonal antibodies (f-MoAb) specific for CD14 (monocytes) and CD61 (platelets). The activated leukocyte assay was performed by incubating another aliquot of the blood samples with f-MoAb specific for CD11b and CD14. All samples were evaluated by means of flow cytometry. Results: We observed significantly more platelet-monocyte aggregates throughout the circulation in patients with CVI than in control subjects (29% vs. 8%; P <.0002). Furthermore, patients with CVI formed significantly more of these aggregates in response to all platelet agonists than did control subjects. There were no significant differences bet
Editeur
Elsevier
Type du document
Conférence : Annual Meeting of the American Venous Forum, 11, Dana Point, Calif, USA, 1999-02-18
Identifiant
PMID : 10550182 ISSN : 0741-5214 CODEN : JVSUES
Source
Journal of vascular surgery A. 1999, vol. 30, n° 5, pp. 844-853 [bibl. : 33 ref.]
Langue
Anglais
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